Author：MeCan Medical–ultrasound machine manufacturers
There are many classifications of nucleic acid extract instruments. In fact, the most commonly used is magnetic bead method. How does this method work? Magnetic pearl nucleic acid extract instrument is generally divided into two types: suction method and magnetic stick method. The specific operation process is as follows:
First, suction method. The extraction of nucleic acids is achieved by fixed magnetic beads and metastasis liquids. Generally, the transfer of the robotic arm is generally controlled by the operating system.
1. Crack. Add a cracking solution to the sample to achieve mixing and adequate reactions of the reaction solution through mechanical exercise and heating, cell cracks, and release nucleic acids.
2. Adsorption. Add magnetic beads to the sample cracking solution and mix it in full. Use magnetic beads to adsorb nucleic acid under high salt and low pH value. Under the action of magnetic fields, the magnetic beads are separated from the solution. Mo the liquid out of the waste liquid tank and abandon the head.
3. Washing. Remove the external magnetic field, add the new suction head to add the washing buffer, mix it fully, remove the impurities, and remove the liquid out of the magnetic field.
4. Requesting. Withdrawing the magnetic field and adding the new suction head to add the elution buffer, fully mixed, the combined nucleic acid is separated from the magnetic bead, so as to obtain a purified nucleic acid.
Second, magnetic stick method. By fixed liquids, the magnetic acid is separated to achieve the separation of nucleic acids. The principle and process are the same as the pumping method. The difference is the method of separation of magnetic beads and liquids. The magnetic stick method is to separate the magnetic beads from the waste liquid through the adsorption of the magnetic rod to the next step to achieve the extraction of nucleic acid.
In fact, the characteristics of this magnetic pearl nucleic acid extract instrument is that it can achieve automation, high -throughput operations, safe and reliable operations, high efficiency, and pollution -free. Therefore, it is recognized by many buyers. When choosing a nucleic acid extraction instrument equipment, no matter which type of device is selected, you must have a basic understanding of it. You cannot judge the quality of the product from the price alone. For more product details, you can consult: 400-6656 -888.