What Are the Steps of Nucleic Acid Detection and What Are the Equipment for Extracting Nucleic Acids

  Author:MeCan Medical–ultrasound machine manufacturers

Nucleic acid is the carrier of genetic information, an important biological information molecule, and the main object of molecular biology research. Nucleic acid extraction includes DNA extraction, RNA extraction, and plasmid extraction. Regardless of the structure of nucleic acid or functional research, first of all, nucleic acids need to be extracted and purified. Therefore, nucleic acid extraction is an important and basic operation in molecular biology experimental technology. Under the epidemic, the demand for nucleic acid detection is increasing day by day.

At present, most of the new coronary virus nucleic acid detection products are mostly adopted by the "real-time fluorescence RT-PCR detection" method. RT-PCR requires two steps for nucleic acid extraction and amplification. The product is mainly divided into two steps during the detection process, namely nucleic acid extraction and amplification detection. Nucleic acid extraction is the starting point of downstream nucleic acid testing, research or product development. The quality and integrity of the separated nucleic acid directly affect the research or diagnosis results.

Magnetic pearl nucleic acid extraction instrument is a simple and efficient medical equipment that extract nucleic acid today. Magnetic particles can be removed by permanent magnet in the magnetic field. These magnetic particles can be made of iron oxide particles of the active group. Due to the large surface area and strong ability to combine nucleic acids, it can be used as a better separation carrier. If the container's side wall magnetism is given, the magnetic beads with nucleic acids in the sample mixture are gathered to the container wall, and the container can directly remove other impurities to avoid repeated centrifugation. Magnetic beads are adsorbed by the active group that can be specificly adsorbed.

The production of high -throughput and automated nucleic acid extraction methods reduces genetic testing labor costs and makes large number of detection a reality. The magnetic bead method does not require centrifugal, no need to add multiple reagents, simple operation, and automated operations ensure stable experimental results, avoid differences and errors caused by manual operations, and easily meet the standardization of nucleic acid extraction, which meets the requirements of nucleic acid automation extraction requirements. It is an important direction for the development of nucleic acid purification methods.

What Are the Steps of Nucleic Acid Detection and What Are the Equipment for Extracting Nucleic Acids 1

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